Anti-IGF-IR (Ab-1) Mouse mAb (aIR3)

Application

Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1 µg/sample)r>Neutralization Studies (1 µg/ml; see application references)r>Paraffin Sections (not recommended)r>Immunoblotting (not recommended)

General description

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in IF, IP, Neutralization Studies, Paraffin Sections, Immunoblotting for the detection of IGF-IR (Ab-1).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.

Roth, R. 1988. Science239, 1269.Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.Rosen, O.M. 1987. Science257, 1452.Rechler, M.M., and Nissley, S.P. 1986. Hormone Res.24, 152.Ullrich, A., et al. 1986. EMBO J.5, 2503.Zapf, S., and Froesch, E.R. 1986. Hormone Res.24, 121.Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).Kull, F.C., et al.1983. J. Biol. Chem.258, 6561.

Packaging

100 µg in Plastic ampoule

Physical form

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .

Reconstitution

Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.

Warning

Toxicity: Standard Handling (A)